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I have learned mathematical apparatus necessary for an analysis of complex physical phenomena with special emphasis on biological applications such as spectroscopy, NMR, crystallography etc.
I have contributed to a construction of an instrument for a measurement of Raman Optical Activity and carried out initial experiments with standard protein molecules.
My project comprises of two major parts: A construction of an expression system for a production of recombinant leptin receptor and Biophysical charactrisation of receptor activation. The first part falls into an isolation of receptor cDNA, its engineering for expression and purification in both mammalian and Baculovirus expression systems and purification of a recombinant protein. In the second part, spectroscopic and microcalorimetric experiments will be carried out to characterise receptor activation.