Control of plant growth by networks of structural biopolymers

 

Plants take shape in a completely different way from animals. Plant cells, unlike animal cells, divide and grow while encased within a strong cell wall. The cell walls of each cell are permanently attached to the walls of the cells round about. The growth of a plant is therefore more or less the growth of its cell-wall network, and that is the level at which plant growth is controlled. The expansion of each cell is driven by the osmotic pressure of the cell contents, but the rate and direction in which it expands is determined by the extensibility of the cell wall.

 

Plant cell walls are biocomposites, with a resemblance to fibreglass at the engineering level. Cellulose fibres provide much of their strength. The direction in which the cell grows is normally at right angles to the dominant orientation of cellulose, and the rate of expansion depends on how well the non-cellulosic matrix allows the cellulose fibres to move apart or slide past one another. This is dependent on enzyme action as well as the nanometre-scale geometry of the structure.

 

We are now beginning to understand how the geometry works in wood, using a combination of mechanical experiments, solid-state NMR and vibrational spectroscopy and polymer modelling. By the time plant cells become recognisable as woody, though, they have ceased to grow. Only growing cell walls contain the enzyme activity that allows them to stretch without loss of strength. The aim of this studentship is to extend these studies to growing cell walls

 

It is expected that most of these experiments will be done on seedlings of sunflower, cucumber and the experimental plant Arabidopsis, although it may be possible to adapt the for tree species if the student prefers. Many of the experiments will be carried out on an FTIR microscope and will require some manipulative skill. The mathematical depth in which the project is developed depends very much on the student concerned.