Aleks' Protein Crystallography Home Page
Aleksander W. Roszak, Ph.D.
Department of Chemistry
University of Glasgow
University Avenue
Glasgow G12 8QQ
Scotland, UK
Phone: +44-(0)141-330 4476 or (last 4 digits) 6180 or 6449
Fax: +44-(0)141-330 4888
Email: aleks@chem.gla.ac.uk
Purple Photosynthetic Bacteria: Light-Harvesting Apparatus
- "The structure and function of bacterial light-harvesting complexes" (review), Mol. Membrane Biol. (2004) 21, 183-191 (pdf)
The RC-LH1 Core Complex from Rhodopseudomonas palustris
- "Crystal Structure of the RC-LH1 Core Complex from Rhodopseudomonas palustris", Science (2003) 302, 1969-1972 (pdf)
- Supporting Material for the Science paper (pdf)
- Overall View of the RC-LH1 Core Complex (created with program RIBBONS)
- The RC-LH1 Core Complex from Rhodopseudomonas palustris; PDB entry 1PYH
Reaction Centre from Rhodobacter sphaeroides: Phospholipid Binding Sites
- "Brominated Lipids Identify Lipid Binding Sites on the Surface of the Reaction Center from Rhodobacter sphaeroides", Biochemistry (2007) 46,
2909-2916 (pdf)
Reaction Centre from Rhodobacter sphaeroides: Carotenoid Binding Site
- "Protein Regulation of Carotenoid Binding: Gatekeeper and Locking Amino Acid Residues in Reaction Centres of Rhodobacter sphaeroides", Structure (2004) 12, 765-773 (pdf)
- Carotenoid Binding Site, view 1 (created with PyMOL, DeLano Scientific, 2004)
- Carotenoid Binding Site, view 2 (created with PyMOL, DeLano Scientific, 2004)
- Carotenoid Binding Site, view 3 (created with PyMOL, DeLano Scientific, 2004)
Protein Data Bank entries:
- Structure of the Photosynthetic Reaction Centre from Rhodobacter sphaeroides Carotenoidless Strain R-26.1; PDB entry 1RG5
- Reaction Centre from Rhodobacter sphaeroides Carotenoidless Strain R-26.1 Reconstituted with Spheroidene; PDB entry 1RGN
- Reaction Centre from Rhodobacter sphaeroides Carotenoidless Strain R-26.1 Reconstituted with 3,4-Dihydrospheroidene; PDB entry 1RQK
Reaction Centre from Rhodobacter sphaeroides: Protein-Lipid Interactions
- "Protein-lipid interactions in the purple bacterial reaction centre" (review), Biochim. Biophys. Acta (2002) 1565, 206-214 (pdf)
- "Probing the interface between membrane proteins and membrane lipids by X-ray crystallography" (review), TiBS (2001) 26(2), 106-112 (pdf)
Mutants of the Reaction Centre from Rhodobacter sphaeroides
- "Tuning of the optical and electrochemical properties of the primary donor bacteriochlorophylls in the reaction centre from Rhodobacter sphaeroides: spectroscopy and structure", Biochim. Biophys. Acta (2002) 1554, 75-93 (pdf)
- Overall View of the Reaction Centre
- Some details of the structure of RC, for more info see Richard Cogdell link at the bottom of the page
Structural Studies of Type II Dehydroquinases
- "The Structure and Mechanism of the Type II Dehydroquinase from Streptomyces coelicolor", Structure (2002) 10, 493-503 (pdf)
- "Specifity of substrate recognition by type II dehydroquinases as revealed by binding of polyanions", FEBS Letters (2002) 530, 24-30 (pdf)
- "Experiences with the shikimate-pathway enzymes as targets for rational drug design", Biochem. Soc. Trans. (2003) 31, 548-552 (pdf)
- "The fluoro analogue of the enolate intermediate in the reaction catalyzed by type II dehydroquinases", Org. Biomol. Chem (2004) 2, 1592-1596 (pdf)
- Monomer of Streptomyces coelicolor type II dehydroquinase
- Dodecamer viewed along the NCS-2fold axis
Protein Data Bank entries:
- Crystal Structure of Type II Dehydroquinase from Streptomyces coelicolor Complexed with Phosphate Ions; PDB entry 1D0I
- Structure of Streptomyces coelicolor Type II Dehydroquinase R23A Mutant in Complex with Dehydroshikimate; PDB entry 1GTZ
- Type II Dehydroquinase from Streptomyces coelicolor Complexed with 2,3-Anhydro-Quinic Acid; PDB entry 1GU1
- Apo form of the Type II Dehydroquinase from Streptomyces coelicolor; PDB entry 1GU0
- Type II 3-Dehydroquinate Dehydratase from Mycobacterium tuberculosis in Complex with Sulphate; PDB entry 1H05
- Type II Dehydroquinase from Mycobacterium tuberculosis Complexed with 2,3-Anhydro-Quinic Acid; PDB entry 1H0R
- 3-Dehydroquinate Dehydratase from Mycobacterium tuberculosis in Complex with 3-Hydroxyimino-Quinic Acid; PDB entry 1H0S
- Type II Dehydroquinase from Bacillus subtilis; PDB entry 1GQO
3D-structure of Shikimate Dehydrogenase
- "Structures of Shikimate Dehydrogenase AroE and Its Paralog YdiB", J. Biol. Chem. (2003) 278, 19463-19472 (pdf)
- Crystal Structure of Shikimate Dehydrogenase AroE; PDB entry 1NYT
Collaboration with the Department of Electronics
- "High-Throughput Screens for Postgenomics: Studies of Protein Crystallization Using Microsystems Technology", Anal. Chem. (2002) 74, 3505-3510 (pdf)
Fructose 1,6-Bisphosphatase
- "Structure of rabbit liver fructose 1,6-bisphosphatase at 2.3A resolution", Acta Cryst.(1999) D55, 93-102 (pdf)
- Crystal Structure of Rabbit Liver Fructose-1,6-Bisphosphatase; PDB entry 1BK4
X-ray Equipment Related Stuff
- Manual on the use of the X-ray equipment at the Glasgow Biomedical Research Centre, University of Glasgow
- Mar Research: Image Plates and CCD Detector
- Oxford Cryosystem: Cryostream Cooler Troubleshooting
- Protein Crystallography in SRS at Daresbury
- ADSC Quantum-4 CCD-detector. Basic principles and specs.
Various links
- The HKL Manual. Denzo, XDisplayF, Scalepack. Edition 5 revised Sept. 1998
- HKL Home Page
- CCP4 Program Documentation
- QUANTA Help
- Protein Data Bank
- PDBsum, Ligands, HetGroups, etc.
- SRS Daresbury, PX Stations, Schedules
- ESRF Grenoble, Beamlines
- Hampton Research Home Page. Crystallisation Research Tools. 2000 Catalog
- A Beginner's Guide to HTML
Where to find me:
- Protein Crystallography Group, Glasgow Biomedical Research Centre, University of Glasgow
- Department of Chemistry, University of Glasgow
- Biochemistry: Richard Cogdell Group, IBLS at GBRC, University of Glasgow
Aleks Roszak
Last modified: June 13, 2007
