Joanna Jakus

PhD Research student, Biophysical Chemistry, 2000-2004

Department of Chemistry
Glasgow University
Glasgow G12 8QQ, Scotland, U.K.
Tel: +44-141-330 6566
Fax: +44-141-330 2910
E-mail: j.jakus@chem.gla.ac.uk

The aim of the work is to investigate changes in expansivity and volume accompanying protein unfolding by using different model proteins (such as ubiquitin, lysozyme etc.). By using methanol - water mixtures the solvent ordering effect can be decreased, thus insight into protein solvation under these specific conditions can be gained and the importance of hydrophobic interaction in stabilising protein folded states investigated.

• Heat Capacity (C_p)Changes for processes involving proteins
  
• Thermodynamic description of protein folding-unfolding transition
  
• Noncovalent interactions that determine protein stability
  
• Volumetric properties and solvation of proteins
  
• Alcohols as cosolvents
  
• Pressure Perturbation Calorimetry