Single-molecule techniques reveal details about molecular systems that are otherwise obscured by conventional ensemble methods. They are powerful tools for the study of systems that cannot be synchronised, display static or dynamic heterogeneity, have transient intermediates or undergo rare events. Furthermore, the detection of individual molecules represents the ultimate level of sensitivity for sensors and diagnostics. Fluorescence-based methods have proven to be particularly suitable for single-molecule studies. We are developing and applying single-molecule methods in Glasgow. An underlying theme is the use of single molecule spectroscopy and imaging to probe the structure, dynamics and reactions of DNA.
DNA structure, dynamics and interactions
Research is focused on the structure, dynamics and reactivity of branched DNA molecules.
Multiphoton excitation of fluorescent nucleobases
We are developing new single-molecule methods for the detection of nucleic acids. 